What is the correct order of Hgb migration on agarose or cellulose acetate at pH 8.6?

What is the correct order of Hgb migration on agarose or cellulose acetate at pH 8.6?

• A. C→S→F→A
• B. S→C→A→F
• C. C→S→A→F
• D. S→F→A→C

Answer: (C)

At alkaline pH 8.6, proteins migrate in electrophoresis primarily by their net negative charge: the more negative the hemoglobin, the faster it moves toward the anode. The beta-chain substitutions in Hb C (Glu to Lys) add positive charge, reducing overall negativity; Hb S (Glu to Val) removes a negative charge; Hb A is the normal reference with standard negative charge; Hb F (gamma chains) presents the greatest negative surface charge among common variants. So the movement progresses from least to greatest toward the anode as C, then S, then A, with F moving the farthest.

At alkaline pH 8.6, proteins migrate in electrophoresis primarily by their net negative charge: the more negative the hemoglobin, the faster it moves toward the anode. The beta-chain substitutions in Hb C (Glu to Lys) add positive charge, reducing overall negativity; Hb S (Glu to Val) removes a negative charge; Hb A is the normal reference with standard negative charge; Hb F (gamma chains) presents the greatest negative surface charge among common variants. So the movement progresses from least to greatest toward the anode as C, then S, then A, with F moving the farthest.